Train drivers on the Victoria line are to stage strike action between 9pm on Tuesday night and 3am Wednesday morning.
RMT union members are striking over what general secretary Bob Crow referred to as a “catalogue of complaints” over working conditions and a "culture of bullying and harassment".
London Underground (LU) said it felt it had resolved the matters raised by the union at a meeting held on September 11.
Peter McNaught, LU operations director, said: "If the union feels there are still issues to discuss then they should keep talking to us so that things can be resolved properly."
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